The prevalence of Escherichia coli O157:H7 and Salmonella in frozen and fresh goat meat The demand for goat meat is on the rise in the U.S. A common concern in the production and harvest of animals for human consumption is the presence of foodborne pathog
DOI:
https://doi.org/10.47611/jsr.v10i4.1424Keywords:
contamination, Escherichia coli, Salmonella, Refrigeration, Freezing, MacConkey Agar, Goat meatAbstract
The demand for goat meat is on the rise in the U.S. A common concern in the production and harvest of animals for human consumption is the presence of foodborne pathogens, Escherichia coli O157:H7 and Salmonella. As an “under-researched food vehicle”, there are minimal strategies for the control and prevention of foodborne pathogens with goat meat. Freezing can be an effective way of preventing microbial growth in meat, but it does not eradicate bacteria. Five samples of fresh and frozen goat meat, respectively, were collected and utilized in prevalence determination and microbial quality comparison. Using selective and differential media, 25 grams from each sample were tested for both E. coli O157:H7 and Salmonella. Three of five frozen samples contained E. coli O157:H7 suspects (p = 0.08377); whereas no E. coli O157:H7 suspects were detected with the fresh samples. Two fresh samples and one frozen sample were indicative of the presence of Salmonella. Frozen samples were more heavily contaminated than fresh samples, but overall, there was a low prevalence of these bacteria in the two meat types. These findings could be further substantiated with the inclusion of larger sample size and more confirmative tests.
Downloads
Metrics
References or Bibliography
Barkocy-Gallagher, G. A., Arthur, T. M., Rivera-Betancourt, M., Nou, X., Shackelford, S. D., Wheeler, T. L., & Koohmaraie, M. (2003). Seasonal prevalence of Shiga toxin-producing Escherichia coli, including O157:H7 and non-O157 serotypes, and Salmonella in commercial beef processing plants. Journal of Food Protection, 66(11), 1978–1986. https://doi.org/10.4315/0362-028x-66.11.1978.
Gerba C. P. (2009). Environmentally Transmitted Pathogens. Environmental Microbiology, 445–484. https://doi.org/10.1016/B978-0-12-370519-8.00022-5.
Healy, J. M., and Bruce, B. B. (2019). Salmonellosis (nontyphoidal) - Chapter 4 - 2020 yellow book. Centers for Disease Control and Prevention. https://wwwnc.cdc.gov/travel/yellowbook/2020/travel-related-infectious-diseases/salmonellosis-nontyphoidal.
ISO 22964, E.N., 2017. 6579-1: 2017. Microbiology of the Food Chain—Horizontal Method for the Detection, Enumeration and Serotyping of Salmonella—Part 1: Detection of Salmonella spp. International Organization for Standardization. Geneva, Switzerland.
Larzábal, M., Da Silva, W. M., Multani, A., Vagnoni, L. E., Moore, D. P., Marin, M. S., Riviere, N. A., Delgado, F. O., Vilte, D. A., Victorica, M. R., Ma, T., Le Guan, L., Talia, P., Cataldi, A., & Cobo, E. R. (2020). Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle. Scientific Reports, 10(1), 21535. https://doi.org/10.1038/s41598-020-78752-x.
March, S.B. & Ratnam, S. (1986). Sorbitol-MacConkey medium for detection of Escherichia
coli O157:H7 associated with hemorrhagic colitis. Journal of Clinical Microbiology, 23, 869–872.
Mohamed, A. D. (2017). The microbiological study of minced meat in some markets in Alexandria with reference to Escherichia coli O157:H7. International Journal of Research -Granthaalayah, 5(9), 26–35. https://doi.org/10.29121/granthaalayah.v5.i9.2017.2194.
Müller, D., Greune, L., Heusipp, G., Karch, H., Fruth, A., Tschäpe, H., & Schmidt, M. A. (2007). Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR. Applied and Environmental Microbiology, 73(10), 3380–3390. https://doi.org/10.1128/AEM.02855-06.
Nataro, J. P., & Kaper, J. B. (1998). Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11(1), 142–201. https://doi.org/10.1128/CMR.11.1.142.
Peterz, M., Wiberg, C., & Norberg, P. (1989). The effect of incubation temperature and magnesium chloride concentration on growth of Salmonella in home-made and in commercially available dehydrated Rappaport-Vassiliadis broths. The Journal of Applied Bacteriology, 66(6), 523–528. https://doi.org/10.1111/j.1365-2672.1989.tb04573.x
R Core Team (2020). R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/.
United States Department of Agriculture, USDA, Food Safety and Inspection Service, FSIS. (2013). Goat from Farm to Table. https://www.fsis.usda.gov/food-safety/safe-food-handling-and-preparation/meat/goat-farm-table.
United States Department of Agriculture, USDA, National Agricultural Statistics Service, NASS. (1992). The Census of Agriculture. http://lib-usda-05.serverfarm.cornell.edu/usda/AgCensusImages/1992/01/51/1574/Table-40.pdf
United States Department of Agriculture, USDA, National Agricultural Statistics Service, NASS. (2019). Sheep and Goats. https://usda.library.cornell.edu/concern/publications/000000018
Published
How to Cite
Issue
Section
Copyright (c) 2022 Jordan Wilson-Smith; Dr. Dana Pollard, Dr. Renita Marshall
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright holder(s) granted JSR a perpetual, non-exclusive license to distriute & display this article.