Examining the Morphological and Physiological Comparisons of OVX Murine Bone vs. Mini Pigs, Rats, and Humans

Authors

  • Shania Jafri Stanford University
  • Karrington Hendrix Stanford University https://orcid.org/0000-0003-3030-186X
  • Pedro Cuevas
  • Igor Pilawski Mentor, Vétérinaires sans Frontìeres Germany, Berlin
  • Jill Helms Mentor, Stanford School of Medicine

DOI:

https://doi.org/10.47611/jsr.v10i3.1311

Keywords:

Osteons, Three R's Principle, Mouse, Rat, Mini Pig, Bone Morphology, Ethics, Haversian Canals, Cellular Assays, Histology, Bone Remodeling

Abstract

Osteons are the structure and the base foundation of the human skeletal system. This cylindrical structure contains blood vessels and nerves that supply the bone matrix in humans. To study bone remodeling and bone diseases, mini pigs and rats have mainly been effective models for humans. This study was conducted to examine mouse bone structures in comparison to human, mini pig, and rat bone structures. This addresses the Three Rs principle of clinical testing on animals and proves that mice should be used as models for humans instead of mini pigs and rats. Many scientists prefer not to use mice as models for studying human bone diseases because it has been suggested that their skeletal systems are morphologically and physiologically different–as seen through aging effects. Because mice are easier to produce and can grow at a faster rate, they are more cost and time efficient to use in labs compared to rats and mini pigs. Aniline blue, Ploton silver, picrosirius red stains and TRAP and ALP cellular assays were conducted to analyze bone structures to compare with humans, mini pigs, and rats. Our data did support the hypothesis as explicit similarities between mice bone and other samples such as rats, mini pig, and human was deduced. This study concluded that there were little limitations present by using murine bone as samples for human when studying bone diseases.

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Author Biographies

Igor Pilawski, Mentor, Vétérinaires sans Frontìeres Germany, Berlin

Igor Pilawski, DVM, PhD

Formerly: Stanford University, School of Medicine, Department of Surgery

Currently: Vétérinaires sans Frontìeres Germany, Berlin

Jill Helms, Mentor, Stanford School of Medicine

Jill Helms, DDS, PhD

Professor of Surgery Division of Plastic and Reconstructive Surgery

Stanford School of Medicine

Published

10-11-2021

How to Cite

Jafri, S., Hendrix, K., Cuevas , P., Pilawski, I., & Helms, J. (2021). Examining the Morphological and Physiological Comparisons of OVX Murine Bone vs. Mini Pigs, Rats, and Humans . Journal of Student Research, 10(3). https://doi.org/10.47611/jsr.v10i3.1311

Issue

Section

Research Articles