Epigenetic Regulation of Nostoc punctiforme ATCC 29133 in Response to Nitrogen Availability

Authors

DOI:

https://doi.org/10.47611/jsr.v10i3.1267

Keywords:

epigenetics, nitrogen cycling, carbon cycling, prokaryotes, cyanobacteria, DNA methylation

Abstract

Here, we report the restriction modification system of Nostoc punctiforme ATCC 29133, along with its methylated genome sequence, under contrasting nitrate availability. Generated methylation profiles revealed increased methylation for key enzymes of assimilatory nitrate reduction, suggesting that Nostoc punctiforme employs DNA methylation to regulate its nitrogen metabolism.

Downloads

Download data is not yet available.

Metrics

Metrics Loading ...

References or Bibliography

Casadesús J, Low D. 2006. Epigenetic gene regulation in the bacterial world. Microbiol Mol Biol Rev 70:830-856. doi: 10.1128/MMBR.00016-06.

Eid J, Fehr A, Gray J, Luong K, Lyle J, Otto G, Peluso P, Rank D, Baybayan P, Bettman B. 2009. Real-time DNA sequencing from single polymerase molecules. Science 323:133-138. doi: 10.1126/science.1162986.

Moraes LE, Blow MJ, Hawley ER, Piao H, Kuo R, Chiniquy J, Shapiro N, Woyke T, Fadel JG, Hess M. 2017. Resequencing and annotation of the Nostoc punctiforme ATTC 29133 genome: facilitating biofuel and high-value chemical production. AMB Express 7:42. doi: 10.1186/s13568-017-0338-9.

Roberts RJ, Vincze T, Posfai J, Macelis, D. 2003. REBASE: restriction enzymes and methyltransferases. Nucleic Acids Res 31:418-420. doi: 10.1093/nar/gkg069.

Meeks JC, Elhai J, Thiel T, Potts M, Larimer F, Lamerdin J, Predki P, Atlas R. 2001. An overview of the genome of Nostoc punctiforme, a multicellular, symbiotic cyanobacterium. Photosynth Res 70:85-106. doi: 10.1023/A:1013840025518.

Chaisson MJ, Tesler G. 2012. Mapping single molecule sequencing reads using basic local alignment with successive refinement (BLASR): application and theory. BMC Bioinformatics 13:238. doi: 10.1186/1471-2105-13-238.

Flusberg BA, Webster DR, Lee JH, Travers KJ, Olivares EC, Clark TA, Korlach J, Turner SW. 2010. Direct detection of DNA methylation during single-molecule, real-time sequencing. Nat Methods 7:461. doi: 10.1038/nmeth.

Clark TA, Murray IA, Morgan RD, Kislyuk AO, Spittle KE, Boitano M, Fomenkov A, Roberts RJ, Korlach J. 2011. Characterization of DNA methyltransferase specificities using single-molecule, real-time DNA sequencing. Nucleic Acids Res 40:e29-e29. doi: 10.1093/nar/gkr1146.

Markowitz VM, Chen IM, Palaniappan K, Chu K, Szeto E, Grechkin Y, Ratner A, Jacob B, Huang J, Williams P, Huntemann M, Anderson I, Mavromatis K, Ivanova NN, Kyrpides NC. 2012. IMG: the Integrated Microbial Genomes database and comparative analysis system. Nucleic Acids Res 40 (Database issue):D115–D122. doi: 10.1093/nar/gkr1044.

Published

10-11-2021

How to Cite

Shaw, C., Brooke, C., Avalos, A., Blow, M., Shapiro, N., Woyke, T., & Hess, M. (2021). Epigenetic Regulation of Nostoc punctiforme ATCC 29133 in Response to Nitrogen Availability . Journal of Student Research, 10(3). https://doi.org/10.47611/jsr.v10i3.1267

Issue

Section

Research Articles