CRISPR/Cas9 Gene Editing: An Approach to Prevent Hereditary Cancers
DOI:
https://doi.org/10.47611/jsrhs.v13i2.6515Keywords:
CRISPR/Cas9, Gene-Editing, Heredity, CancerAbstract
Mutations in oncogenes and tumor-suppressor genes can give rise to a variety of genetic cancers. The use of CRISPR/Cas9 can be applicable in the prevention of these cancers through the knockout of oncogenes and the upregulation of tumor-suppressor genes. This paper proposes this use of the technology by reviewing previous research and successes of CRISPR in treating genetic diseases and the application of those methods to preventing cancer. CRISPR/Cas9 can alter mutated DNA; in the case of oncogenes, CRISPR/Cas9 would restore the cell’s ability to grow and divide, while it would restore the tumor-suppressor gene’s ability to induce apoptosis. This paper also discusses the limitations of this technology, including off-target effects and the delivery of CRISPR into cells.
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The Genetics of Cancer - NCI [Internet]. 2015 [cited 2023 Jul 2]. Available from: https://www.cancer.gov/about-cancer/causes-prevention/genetics
Oncogenes, Tumor Suppressor Genes, and DNA Repair Genes [Internet]. [cited 2023 Jul 2]. Available from: https://www.cancer.org/cancer/understanding-cancer/genes-and-cancer/oncogenes-tumor-suppressor-genes.html
Cancer Prevention Recommendations [Internet]. WCRF International. [cited 2023 Oct 29]. Available from: https://www.wcrf.org/diet-activity-and-cancer/cancer-prevention-recommendations/
Vitamins, diet supplements and cancer [Internet]. [cited 2023 Oct 29]. Available from: https://www.cancerresearchuk.org/about-cancer/treatment/complementary-alternative-therapies/individual-therapies/vitamins-diet-supplements
Types of Cancer Treatment - NCI [Internet]. 2017 [cited 2023 Jul 31]. Available from: https://www.cancer.gov/about-cancer/treatment/types
Cancer.Net [Internet]. 2010 [cited 2023 Jul 31]. What is Cancer Survivorship? Available from: https://www.cancer.net/survivorship/what-cancer-survivorship
Yale Medicine [Internet]. [cited 2023 Jul 31]. Side Effects of Cancer Treatment. Available from: https://www.yalemedicine.org/conditions/side-effects-cancer-treatment
Gaj T, Sirk SJ, Shui S lan, Liu J. Genome-Editing Technologies: Principles and Applications. Cold Spring Harb Perspect Biol. 2016 Dec;8(12):a023754.
Broad Institute [Internet]. 2014 [cited 2023 Oct 29]. Questions and Answers about CRISPR. Available from: https://www.broadinstitute.org/what-broad/areas-focus/project-spotlight/questions-and-answers-about-crispr
CRISPR Clinical Trials: A 2023 Update [Internet]. Innovative Genomics Institute (IGI). [cited 2023 Aug 1]. Available from: https://innovativegenomics.org/news/crispr-clinical-trials-2023/
CAR T-cell Therapy and Its Side Effects [Internet]. [cited 2023 Aug 1]. Available from: https://www.cancer.org/cancer/managing-cancer/treatment-types/immunotherapy/car-t-cell1.html
Hart JR, Zhang Y, Liao L, Ueno L, Du L, Jonkers M, et al. The butterfly effect in cancer: A single base mutation can remodel the cell. Proc Natl Acad Sci. 2015 Jan 27;112(4):1131–6.
Antoniou P, Miccio A, Brusson M. Base and Prime Editing Technologies for Blood Disorders. Front Genome Ed [Internet]. 2021 [cited 2023 Aug 1];3. Available from: https://www.frontiersin.org/articles/10.3389/fgeed.2021.618406
Cannan WJ, Pederson DS. Mechanisms and Consequences of Double-strand DNA Break Formation in Chromatin. J Cell Physiol. 2016 Jan;231(1):3–14.
Genome.gov [Internet]. [cited 2023 Aug 15]. Oncogene. Available from: https://www.genome.gov/genetics-glossary/Oncogene
Proto-oncogenes to Oncogenes to Cancer | Learn Science at Scitable [Internet]. [cited 2023 Aug 15]. Available from: https://www.nature.com/scitable/topicpage/proto-oncogenes-to-oncogenes-to-cancer-883/#
Dai B, Yan S, Lara-Guerra H, Kawashima H, Sakai R, Jayachandran G, et al. Data from: Exogenous restoration of TUSC2 expression induces responsiveness to erlotinib in wildtype epidermal growth factor receptor (EGFR) lung cancer cells through context specific pathways resulting in enhanced therapeutic efficacy [Internet]. Dryad; 2016 [cited 2023 Sep 11]. p. 3607180 bytes. Available from: https://datadryad.org/stash/dataset/doi:10.5061/dryad.j151j
Koo T, Yoon AR, Cho HY, Bae S, Yun CO, Kim JS. Selective disruption of an oncogenic mutant allele by CRISPR/Cas9 induces efficient tumor regression. Nucleic Acids Res. 2017 Jul 27;45(13):7897–908.
Association AL. KRAS and Lung Cancer [Internet]. [cited 2023 Aug 31]. Available from: https://www.lung.org/lung-health-diseases/lung-disease-lookup/lung-cancer/symptoms-diagnosis/biomarker-testing/kras
Tang KJ, Constanzo J, Venkateswaran N, Melegari M, Ilcheva M, Morales JC, et al. Focal Adhesion Kinase regulates the DNA damage response and its inhibition radiosensitizes mutant KRAS lung cancer. Clin Cancer Res Off J Am Assoc Cancer Res. 2016 Dec 1;22(23):5851–63.
Liao L, Song M, Li X, Tang L, Zhang T, Zhang L, et al. E3 Ubiquitin Ligase UBR5 Drives the Growth and Metastasis of Triple-Negative Breast Cancer. Cancer Res. 2017 Apr 13;77(8):2090–101.
Joyce C, Rayi A, Kasi A. Tumor-Suppressor Genes. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2023 [cited 2023 Sep 19]. Available from: http://www.ncbi.nlm.nih.gov/books/NBK532243/
Zuckermann M, Hovestadt V, Knobbe-Thomsen CB, Zapatka M, Northcott PA, Schramm K, et al. Somatic CRISPR/Cas9-mediated tumour suppressor disruption enables versatile brain tumour modelling. Nat Commun. 2015 Jun 11;6:7391.
Moses C, Nugent F, Waryah CB, Garcia-Bloj B, Harvey AR, Blancafort P. Activating PTEN Tumor Suppressor Expression with the CRISPR/dCas9 System. Mol Ther Nucleic Acids. 2018 Dec 14;14:287–300.
Cleveland Clinic [Internet]. [cited 2023 Sep 23]. Tumor Suppressor Genes: Your protective cancer shield. Available from: https://my.clevelandclinic.org/health/body/24833-tumor-suppressor-genes
ENSG00000139687 | ICGC Data Portal [Internet]. [cited 2023 Sep 23]. Available from: https://dcc.icgc.org/genes/ENSG00000139687/mutations?mutations=%7B%22from%22:31%7D
ENSG00000012048 | ICGC Data Portal [Internet]. [cited 2023 Sep 23]. Available from: https://dcc.icgc.org/genes/ENSG00000012048/mutations?mutations=%7B%22from%22:21%7D
americord. Prenatal Testing: Facts, Types, Pros & Cons | Americord® [Internet]. 2018 [cited 2023 Oct 8]. Available from: https://www.americordblood.com/prenatal-testing/
Newborn Screening Portal | CDC [Internet]. 2021 [cited 2023 Oct 8]. Available from: https://www.cdc.gov/newbornscreening/index.html
Predictive testing [Internet]. Genomics Education Programme. [cited 2023 Oct 8]. Available from: https://www.genomicseducation.hee.nhs.uk/glossary/predictive-testing/
Guo C, Ma X, Gao F, Guo Y. Off-target effects in CRISPR/Cas9 gene editing. Front Bioeng Biotechnol. 2023 Mar 9;11:1143157.
Uddin F, Rudin CM, Sen T. CRISPR Gene Therapy: Applications, Limitations, and Implications for the Future. Front Oncol. 2020 Aug 7;10:1387.
Byrne BJ, Corti M, Muntoni F. Considerations for Systemic Use of Gene Therapy. Mol Ther. 2021 Feb 3;29(2):422–3.
Zuccaro MV, Xu J, Mitchell C, Marin D, Zimmerman R, Rana B, et al. Allele-Specific Chromosome Removal after Cas9 Cleavage in Human Embryos. Cell. 2020 Dec 10;183(6):1650-1664.e15.
Maani N, Karolczak S, Dowling JJ. Genetic therapy for congenital myopathies. Curr Opin Neurol. 2021 Oct 1;34(5):727–37.
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