Novel T4 bacteriophage and royal jelly infused disinfectant versus E. coli compared to QACs
DOI:
https://doi.org/10.47611/jsrhs.v12i3.5031Keywords:
Antimicrobial Resistance, Quaternary Ammonium Compounds, T4 bacteriophage, Royal Jelly, Major Royal Jelly Proteins, Kirby Bauer Disk Diffusion AssayAbstract
Antimicrobial resistance is an increasingly worsening phenomenon in the 21st century having resulted in thousands of deaths per year. Increases in the diffusion of antimicrobial resistance in gram-negative bacteria and prevalence of specific genes leading to resistance have been linked to an excessive usage of quaternary ammonium compounds (QACs) in gram-negative bacteria such as Escherichia coli. This experiment aims to develop a novel disinfectant solution (T4MRJP) that utilizes the entero-bacteriophage t4 and nine major royal jelly proteins (MRJPs) to inhibit the growth of E. coli on a MHA (Mueller Hinton Agar) growth medium. In the experiment, the Kirby Bauer Disk Diffusion Assay was first applied on six MHA plates inoculated with 36 evenly distributed susceptibility disks containing different concentrations of solution for three QAC groups, a positive t4 bacteriophage group, and two T4 groups at 90% and 98% dilution respectively. Results demonstrated a strong positive correlation between the increases of concentration to demonstrated inhibition but a lack of statistical significance between the T4 and QAC groups in regards to the proposed hypothesis. However, the diluted T4 group was just as effective as the QAC groups at inhibiting bacterial growth, especially at higher concentrations of the administered solution. In a second trial, however, the T4MRJP (Major royal jelly protein) cocktail was significantly less effective than the experimented QAC groups compared to the bacteriophage alone.
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Prestinaci F, Pezzotti P, Pantosti A. Antimicrobial resistance: a global multifaceted phenomenon. Pathogens and Global Health. 2015;109(7):309-318. doi:https://doi.org/10.1179/2047773215y.0000000030
CDC. What Exactly is Antibiotic Resistance? Centers for Disease Control and Prevention. Published July 21, 2022. https://www.cdc.gov/drugresistance/about.html#:~:text=Antimicrobial%20resistance%20is%20an%20urgent
CDC. COVID-19 & Antibiotic Resistance | CDC. www.cdc.gov. Published June 8, 2021. https://www.cdc.gov/drugresistance/covid19.html
Dewey HM, Jones JM, Keating MR, Budhathoki-Uprety J. Increased Use of Disinfectants During the COVID-19 Pandemic and Its Potential Impacts on Health and Safety. ACS Chemical Health & Safety. 2021;29(1). doi:https://doi.org/10.1021/acs.chas.1c00026
Cervinkova D, Babak V, Marosevic D, Kubikova I, Jaglic Z. The Role of theqacAGene in Mediating Resistance to Quaternary Ammonium Compounds. Microbial Drug Resistance. 2013;19(3):160-167. doi:https://doi.org/10.1089/mdr.2012.0154
Jennings MC, Minbiole KPC, Wuest WM. Quaternary Ammonium Compounds: An Antimicrobial Mainstay and Platform for Innovation to Address Bacterial Resistance. ACS Infectious Diseases. 2015;1(7):288-303. doi:https://doi.org/10.1021/acsinfecdis.5b00047
Zou L, Meng J, McDermott PF, et al. Presence of disinfectant resistance genes in Escherichia coli isolated from retail meats in the USA. Journal of Antimicrobial Chemotherapy. 2014;69(10):2644-2649. doi:https://doi.org/10.1093/jac/dku197
Yap ML, Rossmann MG. Structure and function of bacteriophage T4. Future Microbiology. 2014;9(12):1319-1327. doi:https://doi.org/10.2217/fmb.14.91
Fratini F, Cilia G, Mancini S, Felicioli A. Royal Jelly: An ancient remedy with remarkable antibacterial properties. Microbiological Research. 2016;192:130-141. doi:https://doi.org/10.1016/j.micres.2016.06.007
Han Y, Zhou ZC, Zhu L, et al. The impact and mechanism of quaternary ammonium compounds on the transmission of antibiotic resistance genes. Environmental Science and Pollution Research. 2019;26(27):28352-28360. doi:https://doi.org/10.1007/s11356-019-05673-2
Wang Y, Yu Z, Ding P, et al. Non-antibiotic pharmaceuticals promote conjugative plasmid transfer at a community-wide level. Microbiome. 2022;10(1). doi:https://doi.org/10.1186/s40168-022-01314-y
.
Gahongayire S, Almustapha Aliero A, Drago Kato C, Namatovu A. Prevalence and Detection of qac Genes from Disinfectant-Resistant Staphylococcus aureus Isolated from Salon Tools in Ishaka Town, Bushenyi District of Uganda. Canadian Journal of Infectious Diseases and Medical Microbiology. 2020;2020:e1470915. doi:https://doi.org/10.1155/2020/1470915
Ibrahim WA, Marouf SA, Erfan AM, Nasef SA, El Jakee JK. The occurrence of disinfectant and antibiotic-resistant genes in Escherichia coli isolated from chickens in Egypt. Veterinary World. 2019;12(1):141-145. doi:https://doi.org/10.14202/vetworld.2019.141-145
Golec P, Karczewska-Golec J, Łoś M, Węgrzyn G. Bacteriophage T4 can produce progeny virions in extremely slowly growing Escherichia coli host: comparison of a mathematical model with the experimental data. FEMS Microbiology Letters. 2014;351(2):156-161. doi:https://doi.org/10.1111/1574-6968.12372
Bragg R, van der Westhuizen W, Lee JY, Coetsee E, Boucher C. Bacteriophages as potential treatment option for antibiotic resistant bacteria. Advances in experimental medicine and biology. 2014;807:97-110. doi:https://doi.org/10.1007/978-81-322-1777-0_7
Rodriguez JM, Woodworth BA, Horne B, Fackler J, Brownstein MJ. Case Report: successful use of phage therapy in refractory MRSA chronic rhinosinusitis. International Journal of Infectious Diseases. 2022;121:14-16. doi:https://doi.org/10.1016/j.ijid.2022.04.049
Ahiwale S, Tamboli N, Thorat K, Kulkarni R, Ackermann H, Kapadnis B. In Vitro Management of Hospital Pseudomonas aeruginosa Biofilm Using Indigenous T7-Like Lytic Phage. Current Microbiology. 2010;62(2):335-340. doi:https://doi.org/10.1007/s00284-010-9710-6
Dissanayake U, Ukhanova M, Moye ZD, Sulakvelidze A, Mai V. Bacteriophages Reduce Pathogenic Escherichia coli Counts in Mice Without Distorting Gut Microbiota. Frontiers in Microbiology. 2019;10. doi:https://doi.org/10.3389/fmicb.2019.01984
Lisac A, Birsa E, Podgornik A. E. coli biofilm formation and its susceptibility towards bacteriophages studied in a continuously operating mixing – tubular bioreactor system. Microbial Biotechnology. 2022;15(9):2450-2463. doi:https://doi.org/10.1111/1751-7915.14079
Brudzynski K, Sjaarda C. Honey Glycoproteins Containing Antimicrobial Peptides, Jelleins of the Major Royal Jelly Protein 1, Are Responsible for the Cell Wall Lytic and Bactericidal Activities of Honey. Harder J, ed. PLOS ONE. 2015;10(4):e0120238. doi:https://doi.org/10.1371/journal.pone.0120238
Brudzynski K, Sjaarda C, Lannigan R. MRJP1-containing glycoproteins isolated from honey, a novel antibacterial drug candidate with broad spectrum activity against multi-drug resistant clinical isolates. Frontiers in Microbiology. 2015;6. doi:https://doi.org/10.3389/fmicb.2015.00711
Mureşan CI, Dezmirean DS, Marc BD, Suharoschi R, Pop OL, Buttstedt A. Biological properties and activities of major royal jelly proteins and their derived peptides. Journal of Functional Foods. 2022;98:105286. doi:https://doi.org/10.1016/j.jff.2022.105286
Ryan EM, Alkawareek MY, Donnelly RF, Gilmore BF. Synergistic phage-antibiotic combinations for the control of Escherichia coli biofilms in vitro. FEMS Immunology & Medical Microbiology. 2012;65(2):395-398. doi:https://doi.org/10.1111/j.1574-695x.2012.00977.x
Mangieri N, Picozzi C, Cocuzzi R, Foschino R. Evaluation of a Potential Bacteriophage Cocktail for the Control of Shiga-Toxin Producing Escherichia coli in Food. Frontiers in Microbiology. 2020;11. doi:https://doi.org/10.3389/fmicb.2020.01801
Zhang Y, Hu Z. Combined treatment of Pseudomonas aeruginosa biofilms with bacteriophages and chlorine. Biotechnology and Bioengineering. 2012;110(1):286-295. doi:https://doi.org/10.1002/bit.24630
Ramzi A, Oumokhtar B, Ez zoubi Y, Filali Mouatassem T, Benboubker M, El Ouali Lalami A. Evaluation of Antibacterial Activity of Three Quaternary Ammonium Disinfectants on Different Germs Isolated from the Hospital Environment. Gebre AK, ed. BioMed Research International. 2020;2020:1-6. doi:https://doi.org/10.1155/2020/6509740
Rotilie CA, Fass RJ, Prior RB, Perkins RL. Microdilution Technique for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Antimicrobial Agents and Chemotherapy. 1975;7(3):311-315. doi:https://doi.org/10.1128/aac.7.3.311
Loc-Carrillo C, Abedon ST. Pros and cons of phage therapy. Bacteriophage. 2011;1(2):111-114. doi:https://doi.org/10.4161/bact.1.2.14590
Park HG, Kim BY, Park MJ, et al. Antibacterial activity of major royal jelly proteins of the honeybee (Apis mellifera) royal jelly. Journal of Asia-Pacific Entomology. 2019;22(3):737-741. doi:https://doi.org/10.1016/j.aspen.2019.06.005
Kim, Bo Yeon, et al. “Honeybee (Apis Cerana) Major Royal Jelly Protein 4 Exhibits Antimicrobial Activity.” Journal of Asia-Pacific Entomology, vol. 22, no. 1, 1 Mar. 2019, pp. 175–182, www.sciencedirect.com/science/article/pii/S1226861518307581?ref=pdf_download&fr=RR-2&rr=7d00807eb83615cb, https://doi.org/10.1016/j.aspen.2018.12.020.
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