The Effects of Nitrate Pollution on Cellular Regeneration and Survivability in Planarian Flatworms

Authors

  • Zihan Dong Westtown school
  • Jennifer Anthony Westtown School

DOI:

https://doi.org/10.47611/jsrhs.v12i3.4753

Keywords:

Nitrogen pollution, nitrate, planarian, regeneration

Abstract

Increased nitrogen runoff from commercial fertilizer use has raised issues regarding hypoxia in freshwater habitats. One species noted as a water indicator is planarians, known for their pluripotent stem cell regeneration. This study tested the effects of various nitrate concentrations on planarian specimens with ablated heads, to ascertain the effects of the nitrate on regeneration and survivability. The tests found that there is a negative correlation between nitrate concentration and planarian survival rates, proving that nitrogen runoff can indeed adversely affect similar organisms in a freshwater habitat.

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Author Biography

Jennifer Anthony, Westtown School

Westtown School Scientific Research Teacher and Head of Biology Department

References or Bibliography

Abdal Dayem, A., Hossain, M. K., Lee, S. B., Kim, K., Saha, S. K., Yang, G. M., Choi, H. Y., & Cho, S. G. (2017). The Role of Reactive Oxygen Species (ROS) in the Biological Activities of Metallic Nanoparticles. International Journal of Molecular Sciences, 18(1), 120. https://doi.org/10.3390/ijms18010120

Calevro, F., Filippi, C., Deri, P., Albertosi, C., & Batistoni, R. (1998). Toxic effects of aluminium, chromium and cadmium in intact and regenerating freshwater planarians. Chemosphere Vol 34(4), 651-659. https://www.sciencedirect.com/science/article/pii/S0045653598000812

Camargo, J. & Alonso, A. (2006). Ecological and Toxicological Effects of Inorganic Nitrogen Pollution in Aquatic Ecosystems: A Global Assessment. Environment International. 32(6), 831-49. 10.1016/j.envint.2006.05.002.

Cao, Z., Liu, H., Zhao, B., Pang, Q., & Zhang, X. (2020). Extreme Environmental Stress-Induced Biological Responses in the Planarian. BioMed Research International, 2020, 7164230.https://doi.org/10.1155/2020/7164230

Centres for Disease Control and Prevention. (2014, January 16). Nitrate/nitrite toxicity: What are U.S. standards and regulations for nitrates and nitrites exposure? Centres for Disease Control and Prevention. https://www.atsdr.cdc.gov/csem/nitrate-nitrite/standards.html

Cregg, B. (2018, September 20). Alternative sources of nitrogen. MSU Extension.https://www.canr.msu.edu/news/alternative_sources_of_nitrogen

Dean, M., & Duncan, E. M. (2020). Laboratory Maintenance and Propagation of Freshwater Planarians. Current Protocols in Microbiology, 59(1), e120. https://doi.org/10.1002/cpmc.120

Deochand, M. E., Birkholz, T. R., & Beane, W. S. (2016). Temporal regulation of planarian eye regeneration. Regeneration, 3(4), 209–221. https://doi.org/10.1002/reg2.61

Ding, X., Song, L., Han, Y., Wang, Y., Tang, X., Cui, G., & Xu, Z. (2019). Effects of Fe3+ on Acute Toxicity and Regeneration of Planarian (Dugesia japonica) at Different Temperatures. BioMed Research International, 2019, 8591631. https://doi.org/10.1155/2019/8591631

Goel, M. K., Khanna, P., & Kishore, J. (2010). Understanding survival analysis: Kaplan-Meier estimate. International journal of Ayurveda research, 1(4), 274–278. https://doi.org/10.4103/0974-7788.76794

Howarth, R. W. (2008) Coastal nitrogen pollution: A review of sources and trends globally and regionally. Harmful Algae, 2008, 8(1), 14-20. https://www.sciencedirect.com/science/article/pii/S1568988308001078

Rink J. C. (2013). Stem cell systems and regeneration in planaria. Development Genes and Evolution, 223(1-2), 67–84. https://doi.org/10.1007/s00427-012-0426-4.

Thompson, L. J. (2021, April). Nitrate and nitrite poisoning in animals - toxicology. Merck Veterinary Manual. https://www.merckvetmanual.com/toxicology/nitrate-and-nitrite-poisoning/nitrate-and-nitrite-poisoning-in-animals

Published

08-31-2023

How to Cite

Dong, Z., & Anthony, J. (2023). The Effects of Nitrate Pollution on Cellular Regeneration and Survivability in Planarian Flatworms. Journal of Student Research, 12(3). https://doi.org/10.47611/jsrhs.v12i3.4753

Issue

Section

HS Research Projects