Quorum Quenching Potential of Novel Phytochemicals on Chromobacterium violaceum Quorum Sensing
DOI:
https://doi.org/10.47611/jsrhs.v12i2.4325Keywords:
Chromobacterium violaceum, Bacteria, Antimicrobial, Phytochemicals, Quorum Sensing, Quorum Quenching, Antibacterial, Ziziphus mucronataAbstract
The purpose of this project was to evaluate the antibacterial potential of phytochemicals rutin, syringic acid, and Ziziphus mucronata extract on Chromobacterium violaceum quorum sensing. Z. mucronata extracts were imported from South Africa, then extracted in the lab; a SEM imaging series was conducted to evaluate for contamination and further develop a portfolio on it. The null hypothesis was if plant compounds are tested individually, then they will have similar efficiency as current antibiotics; the alternative hypothesis was if plant compounds are tested individually, then they will have greater efficiency than current antibiotics. While the alternative hypothesis was supported by comparing averages, not all of the results had a significant difference to the positive control. The three assays used in the experiment were swarming motility, violacein inhibition, and biofilm inhibition. Throughout the assays, Z. mucronata leaf, and bark extract had the strongest antimicrobial results: both performed statistically stronger than the positive control in at least one assay. In the violacein inhibition assay, Z. mucronata leaf extract had a statistically significant difference to the gentamicin. Additionally, Z. mucronata bark extract had a statistically stronger antibacterial effect to the gentamicin in regards to biofilm inhibition. The results of this project suggest that all of the plant compounds tested had possessed antimicrobial potential as they performed better than the negative control in most assays, but they did not consistently perform stronger than the positive control. The results of this project support the case to further expand on these phytochemicals from an antimicrobial standpoint.
Downloads
References or Bibliography
Acetic Acid, 30% v/v (3+7. (n.d.). LabChem https://www.labchem.com/tools/msds/msds/LC10260.pdf
Al-Majmaie, S, Nahar, L, Sharples, GP, Wadi, K and Sarker, SD (2019) Isolation and antimicrobial activity of rutin and its derivatives from Ruta chalepensis (Rutaceae) growing in Iraq. Records of Natural Products, 13 (1). pp. 64-70. ISSN 1307-6167
Asfour H. Z. (2018). Anti-Quorum Sensing Natural Compounds. Journal of microscopy and ultrastructure, 6(1), 1–10. https://doi.org/10.4103/JMAU.JMAU_10_18
Cheemanapalli Srinivasulu, Mopuri Ramgopal, Golla Ramanjaneyulu, C.M. Anuradha, Chitta Suresh Kumar, Syringic acid (SA) ‒ A Review of Its Occurrence, Biosynthesis, Pharmacological and Industrial Importance, Biomedicine & Pharmacotherapy, Volume 108, 2018, Pages 547-557, ISSN 0753-3322, https://doi.org/10.1016/j.biopha.2018.09.069.
Deryabin, D., Galadzhieva, A., Kosyan, D., & Duskaev, G. (2019). Plant-derived inhibitors of AHL-mediated quorum sensing in bacteria: Modes of action. International Journal of Molecular Sciences, 20(22) doi:http://dx.doi.org/10.3390/ijms20225588
Ghorbani, A., & Esmaeilizadeh, M. (2017). Pharmacological properties of Salvia officinalis and its components. Journal of traditional and complementary medicine, 7(4), 433–440. https://doi.org/10.1016/j.jtcme.2016.12.014
Gloria Gutiérrez-Venegas, Juan Arturo Gómez-Mora, Marco Antonio Meraz-Rodríguez, Mónica Arisbet Flores-Sánchez, Laura Fabiola Ortiz-Miranda, Effect of flavonoids on antimicrobial activity of microorganisms present in dental plaque, Heliyon, Volume 5, Issue 12, 2019, e03013, ISSN 2405-8440, https://doi.org/10.1016/j.heliyon.2019.e03013.
Harrison, Alisha M, and Scott D Soby. "Reclassification of Chromobacterium violaceum ATCC 31532 and its quorum biosensor mutant CV026 to Chromobacterium subtsugae." AMB Express vol. 10,1 202. 7 Nov. 2020, doi:10.1186/s13568-020-01140-1
Kamaeva, A. A., Vasilchenko, A. S., & Deryabin, D. G. (2014). Atomic force microscopy reveals a morphological differentiation of chromobacterium violaceum cells associated with biofilm development and directed by N-hexanoyl-L-homoserine lactone. PLoS One, 9(8) doi:http://dx.doi.org/10.1371/journal.pone.0103741
Kothari, V., Sharma, S., & Padia, D. (n.d.). Recent research advances on Chromobacterium violaceum. Asian Pacific Journal of Tropical Medicine. https://doi.org/10.1016/j.apjtm.2017.07.022
Lee J, Kim JS, Nahm CH, Choi JW, Kim J, Pai SH, Moon KH, Lee K, Chong Y. Two cases of Chromobacterium violaceum infection after injury in a subtropical region. J Clin Microbiol. 1999 Jun;37(6):2068-70. doi: 10.1128/JCM.37.6.2068-2070.1999. PMID: 10325383; PMCID: PMC85035.
Li, X., Zhang, G., Zhu, Y., Bi, J., Hao, H., & Hou, H. (2019). Effect of the gene on AHL-signaling molecules and QS regulatory mechanism in AMB Express, 9(1), 1-11. doi:http://dx.doi.org/10.1186/s13568-019-0917-z
Magdalena Efenberger-Szmechtyk, Agnieszka Nowak & Agata Czyzowska (2021) Plant extracts rich in polyphenols: antibacterial agents and natural preservatives for meat and meat products, Critical Reviews in Food Science and Nutrition, 61:1, 149-178, DOI: 10.1080/10408398.2020.1722060
Material Safety Data Sheet Ethanol, Absolute. (n.d.). ThermoFisher. https://tinyurl.com/5cshmsff
MATERIAL SAFETY DATA SHEET Gentamicin. (n.d.). https://cdn.pfizer.com/pfizercom/products/material_safety_data/PZ00759.pdf
Md, A. H., Seung-Jin, L., Na-Hye Park, Abraham, F. M., Biruk, T. B., Kang, J., . . . Seung-Chun, P. (2017). Impact of phenolic compounds in the acyl homoserine lactone-mediated quorum sensing regulatory pathways. Scientific Reports (Nature Publisher Group), 7, 1-16. doi:http://dx.doi.org/10.1038/s41598-017-10997-5
Mohabi, S., Kalantar-Neyestanaki, D., & Mansouri, S. (2017). Inhibition of quorum sensing-controlled virulence factor production in Pseudomonas aeruginosa by Quercus infectoria gall extracts. Iranian journal of microbiology, 9(1), 26–32.
Mongalo, N. I., Mashele, S. S., & Makhafola, T. J. (2020). Ziziphus mucronata Willd. (Rhamnaceae): it's botany, toxicity, phytochemistry and pharmacological activities. Heliyon, 6(4), e03708. https://doi.org/10.1016/j.heliyon.2020.e03708
Ravichandran, V., Zhong, L., Wang, H., Yu, G., Zhang, Y., & Li, A. (2018). Virtual Screening and Biomolecular Interactions of CviR-Based Quorum Sensing Inhibitors Against Chromobacterium violaceum. Frontiers. https://www.frontiersin.org/articles/10.3389/fcimb.2018.00292/full
SAFETY DATA SHEET. (n.d.). https://tinyurl.com/mryu35r7
SAFETY DATA SHEET Crystal Violet. (n.d.). ThermoFisher. https://tinyurl.com/mthpbu4z
Safety Data Sheet Ethyl Acetate. (n.d.). Thermofisher. https://tinyurl.com/b9vvjpmh
Safety Data Sheet Syringic Acid. (n.d.). https://cdn.caymanchem.com/cdn/msds/19196m.pdf
Silva, Danielle M. et al. Plant Extracts Display Synergism with Different Classes of Antibiotics. Anais da Academia Brasileira de Ciências [online]. 2019, v. 91, n. 02 https://doi.org/10.1590/0001-3765201920180117.
Society for Science and the Public (2021-22). International Science and Engineering Fair 2021-22: International Rules & Guidelines. Washington, DC: Society for Science and the Public. ISEF Rules and Guidelines
South African Journal of Botany. (n.d.). https://doi.org/10.1016/j.sajb.2018.09.010
Stauff, D. L., & Bassler, B. L. (2011). Quorum sensing in Chromobacterium violaceum: DNA recognition and gene regulation by the CviR receptor. Journal of bacteriology, 193(15), 3871–3878. https://doi.org/10.1128/JB.05125-11
Tiwary, B. K., Ghosh, R., Moktan, S., Ranjan, V. K., Dey, P., Choudhury, D., Dutta, S., Deb, D., Das, A. P., & Chakraborty, R. (2017). Prospective bacterial quorum sensing inhibitors from Indian medicinal plant extracts. Letters in applied microbiology, 65(1), 2–10. https://doi.org/10.1111/lam.12748
Yang, M., Meng, F., Gu, W., Li, F., Tao, Y., Zhang, Z., Zhang, F., Yang, X., Li, J., & Yu, J. (2020). Effects of Natural Products on Bacterial Communication and Network-Quorum
Sensing. BioMed research international, 2020, https://doi.org/10.1155/2020/863810
Published
How to Cite
Issue
Section
Copyright (c) 2023 Akansha Mehta; Leya Joykutty, Juliana Carvalho De Arruda Caulkins
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
Copyright holder(s) granted JSR a perpetual, non-exclusive license to distriute & display this article.
