A Microfluidic Chip Designed for Stimulating Drug Diffusion
DOI:
https://doi.org/10.47611/jsrhs.v11i2.2644Keywords:
drug delivery, diffusion, microfluidic chip, cell cultureAbstract
Two-dimensional, three-dimensional or microfluidic chip-based cell cultures have been extensively used to identify effective compounds for drug development in the past few decades. Nevertheless, these traditional strategies of cell cultures are unable to capture the in vivo process of drug administration and diffusion. Here, we exploited the techniques of microfluidic chips and developed a new platform to dynamically simulate drug delivery and diffusion. Using this platform, we designed two experiments to quantify the diffusivity from source channel to target channel: (1) the pH value in target channel regulated by the diffusion of input solution from source channel; (2) the oxygen concentration in target channel regulated by the diffusion of oxygen produced by oxygen-enriched water in source channel. The input channel and the target channel were separated by nanoporous membranes mimicking biological tissue walls. These two experiments as proof-of-concept demonstrated that our platform can simulate the in vivo process of drug diffusion and be applied to study drug diffusivity.
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