Investigating the DNA Damage Response Elicited by Transposable Element Integration
DOI:
https://doi.org/10.47611/jsrhs.v11i2.2544Keywords:
transposable elements, DNA damage response, CRISPR/Cas12kAbstract
All organisms encounter DNA damage daily through UV exposure, carcinogens, and more. Therefore, there must be a conserved system in place to repair such damage. The DNA Damage Response (DDR) is the conserved system that protects and repairs DNA lesions and breaks. It is known that some mobile genetic elements, such as transposable elements (TEs), can elicit the DDR to aid the transposition efficiency while maintaining a low mutagenesis rate. However, other TE and CRISPR/Cas9 studies propose that DDR activation can lead to off target and mutagenic effects. With the search for a better genetic editor, the CRISPR/Cas12k system has become a hot target due to its precise prokaryotic genome editing through transposition. By considering the mechanisms at play in endogenous TEs, retrotransposons, and CRISPR/Cas9, we can achieve a clearer understanding of the eukaryotic cell’s response to genetic modification through the CRISPR/Cas12k system.
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