PFOA and PFOS induced neuronal cell apoptosis via the FADD ligand of the death pathway.
DOI:
https://doi.org/10.47611/jsrhs.v11i1.2483Keywords:
Biology, Environmental, ChemicalsAbstract
Per and polyfluoroalkyl substances (PFAS chemicals) have been prevalent since the 1940s. Yet, the impacts of these synthetic substances on human health still remain uncertain. Both in vivo and in vitro studies exhibit cytotoxicity targets including: hepatotoxicity, reproductive toxicity, thyroid disruption, neurotoxicity, and many more. In this study, 2 specific PFAS chemicals - Perfluoroooctane sulfonate and Perfluorooctanoic acid - were tested for their effects on HTB-11 neuronal cells. Molecular docking was utilized to determine linkages with death ligands. PFOS and PFOA prompted caspase activity on targeted neuronal cells, indicating that these chemicals significantly induce apoptosis. Evidence of high binding affinities with a death ligand was also present. PFOS and PFOA, in addition, reduced target neuronal cell survival in the MTT assay, with all concentrations significant. Based on this evidence, researchers should study more closely the health effects PFAS chemicals can instigate in human bodies.
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