Gene Editing in Life Sciences
DOI:
https://doi.org/10.47611/jsrhs.v10i2.1520Keywords:
CRISPR, Gene Editing, Gene Drives, De-Extinction, Viral Detection, Crop Modification, SHERLOCK, DETECTR, Virus, GMO, Plants, Malaria, Biology, CRISPR ApplicationsAbstract
Gene editing technologies like the CRISPR-Cas9 system have forever changed the way we analyze genetics. As we now have a way to alter genetics, we can now investigate not just how a biological mechanism works, but wonder what we could do to make it better. This paper will discuss how biotechnology and gene editing are integrated into life sciences and biology, where most applications are found. It will be divided into four sections, each addressing how gene editing technology has advanced a field in life science. Firstly, the focus will be on viral detection systems SHERLOCK (Specific High-sensitivity Enzymatic Reporter UnLOCKing) and DETECTR (DNA Endonuclease Targeting CRISPR Trans Reporter), and their importance to the current global pandemic as well as other applications. Then the attention will be diverted into the rapidly popularizing work on gene drives and attempts to drive the evolution of populations of species to benefit mankind. Next, we will discuss the link between biotechnology and species resurrection while discussing ongoing efforts and the ethics of such massive biodiversity shifts. And finally, we discuss possible solutions for the world's hunger crisis using biotechnology with a section on genetically modified crops. I hope to properly explain the growing applications of gene editing by the end of this paper.
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Mysterious Repeated Sequence to Genome Editing Technology. Retrieved October 24, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5847661/
Ma, Y., Zhang, L., & Huang, X. (2014, November 07). Genome modification by CRISPR/Cas9. Retrieved October 24, 2020, from https://febs.onlinelibrary.wiley.com/doi/full/10.1111/febs.13110
Gupta, R., & Musunuru, K. (2014, October). Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9. Retrieved October 24, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4191047/?_ga=2.76177173.16339454.1603061089-601410891.1603061089
Gootenberg, J., Abudayyeh, O., Lee, J., Essletzbichler, P., Dy, A., Joung, J., . . . Zhang, F. (2017, April 28). Nucleic acid detection with CRISPR-Cas13a/C2c2. Retrieved October 24, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526198/
Kellner, M., Koob, J., Gootenberg, J., Abudayyeh, O., & Zhang, F. (2019, October). SHERLOCK: Nucleic acid detection with CRISPR nucleases. Retrieved October 24, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6956564/
Gronowski, A. (2018, November 7). Who or What is SHERLOCK? Retrieved October 31, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6247122/
Broughton, J., Deng, X., Yu, G., Fasching, C., Singh, J., Streithorst, J., . . . Chiu, C. (2020, January 01). Rapid Detection of 2019 Novel Coronavirus SARS-CoV-2 Using a CRISPR-based DETECTR Lateral Flow Assay. Retrieved October 31, 2020, from https://www.medrxiv.org/content/10.1101/2020.03.06.20032334v2
Arora, R., Gupta, K., Vijaykumar, A., & Krishna, S. (2019, January 01). DETECTing Merkel cell Polyomavirus in Merkel Tumours. Retrieved October 31, 2020, from https://www.biorxiv.org/content/10.1101/770537v1
Ågren, J., & Clark, A. (2018, November 15). Selfish genetic elements. Retrieved October 24, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6237296/
Buchman, A., & Akbari, O. (2016, February 15). Cheating evolution: Engineering gene drives to manipulate the fate of wild populations. Retrieved October 24, 2020, from https://www.nature.com/articles/nrg.2015.34
McFarlane, G., Whitelaw, C., & Lillico, S. (2018, January 23). CRISPR-Based Gene Drives for Pest Control. Retrieved October 24, 2020, from https://www.sciencedirect.com/science/article/abs/pii/S0167779917302639
Callies, D. (2019, August 06). The ethical landscape of gene drive research. Retrieved October 24, 2020, from https://onlinelibrary.wiley.com/doi/abs/10.1111/bioe.12640
Novak, B. (2018, November 13). De-Extinction. Retrieved October 24, 2020, from https://www.mdpi.com/2073-4425/9/11/548/htm
Yamagata, K., Nagai, K., Miyamoto, H., Anzai, M., Kato, H., Miyamoto, K., . . . Iritani, A. (2019, March 11). Signs of biological activities of 28,000-year-old mammoth nuclei in mouse oocytes visualized by live-cell imaging. Retrieved October 24, 2020, from https://www.nature.com/articles/s41598-019-40546-1
Raman, R. (2017, October 2). The impact of Genetically Modified (GM) crops in modern agriculture: A review. Retrieved October 24, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790416/
Jaganathan, D., Ramasamy, K., Sellamuthu, G., Jayabalan, S., & Venkataraman, G. (2018, July 17). CRISPR for Crop Improvement: An Update Review. Retrieved October 24, 2020, from https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6056666/
Hug, K. (2008, February 1). Genetically modified organisms: Do the benefits outweigh the risks? Retrieved October 24, 2020, from https://medicina.lsmuni.lt/med/0802/0802-01e.htm
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Copyright (c) 2021 Jeet Patel; Erin Berlew
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