Transfer of Conditionability Between Planaria Through DNA and RNA

Authors

  • Cecilia Schiavoni Marymount School of New York
  • Jessica Genter Marymount School of New York

DOI:

https://doi.org/10.47611/jsrhs.v10i1.1397

Keywords:

memory, conditioning, planaria, invertebrates, RNA, DNA, associative learning

Abstract

This experiment examined whether the transfer of DNA or RNA from conditioned planaria could make the recipients more susceptible to similar conditioning. It has been shown in prior experiments that cephalization is not necessary for memory retention in planaria, so there must be another, non-neural mechanism for memory. Thus, changes in DNA or RNA may be responsible for memory retention, and introducing these changes to naive planaria could transfer the "memory" of the conditioned donor. This study included 3 groups of 11 planaria: the control group, the DNA receival group, and the RNA receival group. After the control group was conditioned, each planarian’s RNA and DNA were extracted and given to its genetically identical counterparts in the DNA and RNA receival groups. The number of trials it took to condition the planaria in each group was measured and compared to see if the transfer of RNA or DNA made the recipients more easily conditionable than the control donor group. The results show that the transfer of RNA and DNA made insignificant changes to the conditionability of recipients. However, further research may be able to identify the location of memory bearing molecules, which, if applied to experiments similar to this one, would ensure that the planaria received a high enough concentration of genetic material for any changes present to make a significant effect.

Downloads

Download data is not yet available.

References or Bibliography

Abbot, S. M., & Wong, G. K. (2008). The conditioning and memory retention of planaria (Dugesia tigrina) for directional preferences. BIOS, 79(4). https://doi.org/10.1893/0005-3155-79.4.160

Bédécarrats, A., Chen, S., Pearce, K., Cai, D., & Glanzman, D. L. (2018). RNA from Trained Aplysia Can Induce an Epigenetic Engram for Long-Term Sensitization in Untrained Aplysia. eNeuro. http://dx.doi.org/10.1523/ENEURO.0038-18.2018

Black Planaria (Dugesia dorotocephala), Culture, Class Size 30. (n.d.). Flinn Scientific. Retrieved May 7, 2020, from https://www.flinnsci.com/black-planaria-dugesia-dorotocephala-culture-class-size-30/lm1096/

Carney, R. S.E. (2018). An Emerging Role for RNA in a Memory-Like Behavioral Effect in Aplysia. ENeuro. https://doi.org/10.1523/ENEURO.0193-18.2018

Hawkins, R. D., & Byrne, J. H. (2015). Associative Learning in Invertebrates. Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/cshperspect.a021709

Mueller, C. T. (2002). The use of classical conditioning in planaria to investigate a non-neuronal memory mechanism. Research Science Institute. http://drmichaellevin.org/Planaria/prelimdata/CMueller.pdf

Ragland, R. S., & Ragland, J. B. (1965). Planaria: Interspecific transfer of a conditionability factor through cannibalism. Psychonomic Science, 3(1-12), 117-118. https://doi.org/10.3758/BF03343051

Shomrat, T., & Levin, M. (2013). An automated training paradigm reveals long-term memory in planarians and its persistence through head regeneration. The Journal of Experimental Biology, 216, 3799-3810. https://doi.org/10.1242/jeb.087809

Smalheiser, N. R., Manev, H., & Costa, E. (2001). RNAi and brain function: Was McConnell on the right track? Trends in Neurosciences, 24(4), 216-218. https://doi.org/10.1016/s0166-2236(00)01739-2

Walker, D. R. (1966). Memory transfer in planarians: An artifact of the experimental variables. Psychonomic Science, 5(9), 357-358. https://doi.org/10.3758/BF03328437

Walker, D. R., & Milton, G. A. (1966). Memory transfer vs. sensitization in cannibal planarians. Psychonomic Science, 5(7), 293-294. https://doi.org/10.3758/BF03328401

Published

03-31-2021

How to Cite

Schiavoni, C., & Genter, J. (2021). Transfer of Conditionability Between Planaria Through DNA and RNA. Journal of Student Research, 10(1). https://doi.org/10.47611/jsrhs.v10i1.1397

Issue

Section

HS Research Projects